PTPN2 Downregulation Is Associated with Albuminuria and Vitamin D Receptor Deficiency in Type 2 Diabetes Mellitus

Figure 4

PTPN2 inhibits inflammatory factors induced by high glucose in THP-1 cells. siCtrl and siPTPN2 THP-1 cells were stimulated with high glucose (30 mmol/L) for 48 hours and then treated with or without paricalcitol (0.2 ng/mL for 6 hours) or recombined human PTPN2 (50 ng/mL for 24 hours). (a, b) PTPN2 and VDR protein levels were determined by Western blot; GAPDH was used as a loading control. (c) PTPN2 and VDR mRNA levels were quantified by real-time RT-PCR. (d) Real-time RT-PCR of inflammatory cytokines MCP-1, IL-6, and TNFα. Experiments were repeated three times, and data represent mean ± SD versus siCtrl (, ), versus siCtrl + HG (, ), versus siCtrl + HG + PTPN2 (, ), and versus HG + siPTPN2 ().

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