MicroRNA-22 Promotes Renal Tubulointerstitial Fibrosis by Targeting PTEN and Suppressing Autophagy in Diabetic Nephropathy

Figure 2

The function of autophagy on HG-induced expression of Col IV and α-SMA in NRK-52E cells. (a) NRK-52E cells were treated with NG or HG for 24 or 48 h; the protein level of Col IV was detected by Western blot. (b) ImageJ densitometric analysis of the protein level of Col IV; data represent mean ± SEM, , , . For (c) and (d), NRK-52E cells were treated with DMSO or 100 nM Rap and NG or HG for 48 h. The expression of LC3-I, LC3-II, p62, and Col IV was detected by Western blot. ImageJ densitometric analysis of the protein levels; data represent mean ± SEM, , , . Rap: rapamycin.

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