The Antidiabetic Agent Sodium Tungstate Induces Abnormal Glycogen Accumulation in Renal Proximal Tubules from Diabetic IRS2-Knockout Mice

Figure 3

Protein expression of the gluconeogenic enzyme PEPCK in the kidney from NaW-IRS2-KO mice. Western blot analysis of PEPCK in the total kidney from untreated WT (WT), treated WT (NaW-WT), untreated IRS2-KO (IRS2-KO), and treated IRS2-KO (NaW-IRS2-KO) mice (a, b). (c–f) Immunofluorescence analysis of PEPCK in the cortex from WT, NaW-WT, IRS2-KO, and NaW-IRS2-KO kidneys. (g) Combined glycogen (red) and PEPCK (green) detection in kidneys from NaW-IRS2-KO mice without α-amylase treatment. DAPI was used for nuclear staining. pt: proximal tubule, npt: nonproximal tubule. Representative images of each group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)