Effects of Type 2 Diabetic Serum on Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells

Figure 4

(a) mRNA expression of osteogenic differentiation-associated genes in different periods of culture, detected with quantitative real-time PCR. Osteogenic differentiation-related genes RUNX2, OPN, and OCN were detected after MSCs were cultured for different periods. mRNA expression increased gradually as the culture time increased. All results were expressed as the and analyzed using one-way ANOVA. Student–Newman–Keuls multiple-range test was used to compare results between two groups: versus culture 7 d, ; versus culture 14 d, . . (b) mRNA expression of osteogenic differentiation-associated genes in MSCs cultured with human sera. Osteogenic differentiation of MSCs was induced by culture with human serum. Cells were cultured in sera containing different concentrations of HbA1c: <6.5%, 6.5%–8.0%, 8.0%–10%, or >10%. mRNA expression was detected when the MSCs had been cultured for about 21 days. Diabetic sera inhibited the expression of osteogenic differentiation-associated genes. All results were expressed as the and analyzed using one-way ANOVA. Student–Newman–Keuls multiple-range test was used to compare results between two groups: versus group below 6.5%, ; versus group 6.5–8%, ; versus group 8–10%, , . .

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