Haptoglobin Genotype Is a Determinant of Hemoglobin Adducts and Vitamin E Content in HDL

Figure 1

Detection of Hb cross-links on HDL by Western blot. Hp 1-1 or 2-2 was incubated with Hb (1 : 1 ratio) creating a Hp-Hb complex. These complexes, with or without H₂O₂ (1 : 10 molar excess), were added to HDL from healthy volunteers, loaded on a SDS-PAGE gel and were transferred to a PVDF membrane. (1) HDL, (2) HDL + H₂O₂, (3) Hb, (4) HDL + Hb, (5) HDL + Hb + H₂O₂, (6) HDL + Hp 1-1, (7) HDL + Hb/Hp 1-1, (8) HDL + Hb/Hp 1-1 + H₂O₂, (9) HDL + Hp 2-2, (10) HDL + Hb/Hp 2-2, and (11) HDL + Hb/Hp 2-2 + H₂O₂.