Sweet Taste Receptors Mediated ROS-NLRP3 Inflammasome Signaling Activation: Implications for Diabetic Nephropathy

Figure 3

High glucose inhibited the expression of STRs and activated NLRP3 inflammasome. (a) GMCs (SV-40 MES 13) and human proximal tubular cells (HK-2) were treated with 30 mmol/L high glucose for 6, 12, and 24 h, and Western blot or qRT-PCR was performed to detect T1R2 and T1R3 protein or mRNA expression levels. (b) Cells were treated with the indicated concentrations of glucose or mannitol for 24 h. Western blot or qRT-PCR was performed to detect T1R2 and T1R3 protein or mRNA expression levels. (c) Western blotting analysis of NLRP3, cleaved caspase-1, and cleaved IL-1β in GMCs treated with an indicated concentration of glucose or mannitol for 24 h. (d) IL-1β protein level in the GMC culture supernatant was determined by ELISA. Data are expressed as mean ± SD. versus NC group.

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