Sweet Taste Receptors Mediated ROS-NLRP3 Inflammasome Signaling Activation: Implications for Diabetic Nephropathy

Figure 4

High glucose-induced downregulation of STRs and associated signaling components was reversed by lactisole. Mouse GMCs (SV-40 MES 13) was firstly incubated with 3, 5, and 10 mM lactisole; then 30 mM high glucose (HG) alone was intervened by the indicated concentrations of lactisole following 30 mM high glucose for 24 h. (a) Cell viability was determined by MTT analysis. (b) The protein and mRNA expression of T1R2 and T1R3 in GMCs were detected by Western blotting and qRT-PCR. (c) The protein expression of T1R2 and T1R3 in human proximal tubular cells (HK-2) was detected by Western blot. (d) STR-associated signaling components (α-gustducin, PLCβ2, and TRPM5) were detected by qRT-PCR. (e) GMCs were stimulated with 30 mmol/L glucose in the presence (blue), and absence (red) of 5 mmol/L lactisole, and the Ca²⁺ influx changes in Ca²⁺ dye fluorescence was monitored. Data are expressed as mean ± SD. versus NC group. versus 30 mM glucose (HG) stimulation group.

(a)

(b)

(c)

(d)

(e)