Sweet Taste Receptors Mediated ROS-NLRP3 Inflammasome Signaling Activation: Implications for Diabetic Nephropathy

Figure 5

High glucose-induced activation of ROS-NLRP3 inflammasome signaling was reversed by lactisole. (a) The fluorescence images (100×) and quantitative assay for intracellular ROS was determined by detection kits. (b) The protein and mRNA expression of NLRP3, cleaved caspase-1, and cleaved IL-1β were detected by Western blotting. (c) IL-1β protein level in the cell culture supernatant was assayed after lactisole treatment. (d) Immunofluorescence and a laser scanning confocal microscope (400×) detected the expressions of NLRP3, caspase-1, and IL-1β in GMCs as green fluorescence that overlapped with blue fluorescence emitted by the nuclear stain DAPI. The values of semiquantitative analysis for average intensity were assessed and the gray graphs confirmed these trends. Data are expressed as mean ± SD. versus NC group. versus 30 mmol/L glucose (HG) stimulation group.

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