TERT and Akt Are Involved in the Par-4-Dependent Apoptosis of Islet β Cells in Type 2 Diabetes

Figure 2

Diabetes up-regulates Par-4, down-regulates TERT, induces islet β cell apoptosis, and decreases the survival rate and glucose-induced insulin secretion. (a) Effect of high-glucose/palmitate treatment for 12 h, 24 h, and 48 h on NIT-1 cells. The control group was treated with DMSO for 48 h. Cells were exposed simultaneously to high glucose (25.5 mmol/L) and palmitate (100 nmol/L) for 12 h, 24 h, or 48 h and then harvested, and cell lysates were prepared. The total cellular lysates were subjected to western blot analysis using antibodies against Par-4 and TERT. (b) Apoptosis was detected in each group of cells via TUNEL staining. Expression of Par-4 (c) and TERT (d), apoptosis rates (e), cell survival rates determined in MTT assays (f), and glucose-stimulated insulin secretion (g) in each group. Compared with the C group, . ^#Compared with the H24 group, .

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