TERT and Akt Are Involved in the Par-4-Dependent Apoptosis of Islet β Cells in Type 2 Diabetes

Figure 7

Par-4 binds directly to TERT. (7.1.1) Interaction between Par-4 and TERT, ligand: Par-4 protein, analyte: TERT-1: TERT (1–183); TERT-2: TERT (170–546); TERT-3: TERT (528–924); and TERT-4: TERT (915–1182). (7.1.2) Interaction between Par-4 and TERT-1, A1: TERT-1; E1: FAP-tag; F1: negative control. (7.1.3) Interaction between Par-4 and TERT-2, A1–E1: different concentrations of TERT-2; F1: FAP-tag; G1: negative control. (7.1.4) Interaction between Par-4 and TERT-3, A1: TERT-3; E1: FAP-tag; F1: negative control. (7.1.5) Interaction between Par-4 and TERT-4, A1: TERT-4; E1: FAP-tag; F1: negative control. (7.2) Interaction between Par-4 and TERT-2 (tag free), ligand: Par-4-1: Par-4 (1–160); Par-4-2: Par-4 (161–340); and Par-4-3: full-length Par-4, analyte: TERT (170–546 tag free): TERT-2 (tag free). (7.2.1) Interaction between Par-4-1 and TERT-2 (tag free), A2–D2: different concentrations of Par-4-1; E2: FAP-tag; F2: negative control. (7.2.3) Interaction between Par-4-2 and TERT-2 (tag free), A3–D3: different concentrations of Par-4-2; E2: FAP-tag; F2: negative control. (7.2.4) Interaction between full-length Par-4 and TERT-2 (tag free), A4–D4: different concentrations of Par-4-2; E2: FAP-tag; F2: negative control.