Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes

Figure 2

Protein expression levels in nondiabetic and diabetic gastric fundus and antrum smooth muscles. Representative immunoblots of (a) enteric γ-actin, 250 ng muscle lysate/lane, (c) MYLK, 1 μg muscle lysate/lane, (e) PP1δ, 2.5 μg muscle lysate/lane, and (g) ROCK2, 2.5 μg muscle lysate/lane, expression in nondiabetic and diabetic fundus (left panel, , each group) and nondiabetic and diabetic antrum muscles (right panel, , each group). (b) Enteric γ-actin expression per g of nondiabetic and diabetic fundus and antrum muscles. (d) Smooth muscle MYLK expression relative to enteric γ-actin expression in nondiabetic and diabetic fundus and antrum smooth muscles. (f) PP1δ expression relative to enteric γ-actin expression in nondiabetic and diabetic fundus and antrum smooth muscles. (h) ROCK2 expression relative to enteric γ-actin expression in nondiabetic and diabetic fundus and antrum smooth muscles. , , and .

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