Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes

Figure 5

MYL9 expression and S19 phosphorylation in nondiabetic and diabetic gastric fundus and gastric antrum smooth muscles. (a) Representative immunoblots of MYL9 expression in nondiabetic and diabetic fundus (left panel, , each group) and nondiabetic and diabetic antrum muscles (right panel, , each group), 500 ng muscle lysate per lane. (b) MYL9 expression relative to enteric γ-actin expression in nondiabetic and diabetic fundus and antrum smooth muscles. (c) Representative immunoblots of phosphorylated S19 in nondiabetic and diabetic fundus (left panel, , each group) and nondiabetic and diabetic antrum muscles (right panel, , each group), 1 μg muscle lysate per lane. (d) S19 phosphorylation relative to MYL9 expression in nondiabetic and diabetic fundus and antrum smooth muscles. , , and .

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