Proteomic Analysis of Hippocampus and Cortex in Streptozotocin-Induced Diabetic Model Mice Showing Dementia

Table 1

Identified proteins in the hippocampus and their functional categories.

Spot no.	Accession no.	Identified protein	Observed Mw (kDa)/pI	Theoretical Mw (Da)/pI	Score	SC	Fold change (STZ/CTL)	ANOVA value
Cytoskeletal protein
9	gi|9506971	Profilin-2	12/6.2	15,364/6.55	145	28	0.8	0.048
12	gi|7106439	Tubulin beta-5 chain	43/5.0	50,095/4.78	295	14	0.8	0.012
16	gi|508538	Alpha-internexin	43/5.6	56,007/5.16	232	14	0.7	0.013
Oxidoreductase
5	gi|3603241	Type II peroxiredoxin 1	21/5.1	21,949/5.20	136	9	1.1	0.032
13	gi|7710012	Ketimine reductase mu-crystallin	32/5.6	33,673/5.44	208	14	0.8	0.002
15	gi|6678674	L-Lactate dehydrogenase B chain isoform 1	32/5.9	36,834/5.70	233	14	0.7	0.041
Protein deubiquitination
8	gi|148705825	Ubiquitin carboxy-terminal hydrolase L1, isoform CRA_a	26/5.3	24,977/5.05	221	21	0.9	0.018
11	gi|261824000	Proteasome subunit alpha type-3	28/5.4	28,615/5.29	201	17	0.8	0.043
Energy metabolism
1	gi|3766201	ATP-specific succinyl-CoA synthetase beta subunit	37/5.7	46,557/5.65	170	8	1.8	0.014
GTPase activation
3	gi|31982030	Rho GDP-dissociation inhibitor 1	26/5.2	23,450/5.12	114	15	1.2	0.038
Heme binding
2	gi|3724328	Heme-binding protein	22/5.1	21,165/5.18	95	14	1.3	0.023
Hydrolase
14	gi|38371755	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1	31/5.8	31/5.8 31,760/5.64	125	8	0.7	0.011
Iron storage
10	gi|6753912	Ferritin heavy chain	18/5.5	21,224/5.53	237	26	0.8	0.039
Neurotransmitter release
7	gi|6678437	Translationally controlled tumor protein	24/4.7	19,564/4.76	98	5	0.9	0.015
Protease inhibitor
4	gi|84794552	Phosphatidylethanolamine-binding protein 1	21/5.1	20,988/5.19	168	25	1.1	0.032
Transcription
6	gi|6679299	Prohibitin	27/5.7	29,859/5.57	270	20	0.9	0.038

Spot numbers correspond to the 2-DE gel in Figure 1(a). Accession numbers were obtained from the National Center for Biotechnology Information (NCBI) database. Score and sequence coverage (SC) were obtained by Mascot database searching (http://www.matrixscience.com). values were obtained by ANOVA, .