Proteomic Analysis of Hippocampus and Cortex in Streptozotocin-Induced Diabetic Model Mice Showing Dementia
Table 2
Identified proteins in the cortex and their functional categories.
Spot no.
Accession no.
Identified protein
Observed Mw (kDa)/pI
Theoretical Mw (Da)/pI
Score
SC
Fold change (STZ/CTL)
ANOVA value
Cytoskeletal protein
17
gi|3642627
Alpha-tubulin, partial
40/6.1
11,058/4.85
70
15
1.4
0.016
18
gi|6678469
Tubulin alpha-1C chain
31/5.6
50,562/4.96
187
10
1.4
0.015
19
gi|49868
Put. beta-actin (aa 27–375)
25/5.7
39,446/5.78
126
8
1.3
0.035
22
gi|809561
Gamma-actin
22/5.6
41,335/5.56
221
10
1.3
0.037
23
gi|148539957
Alpha-internexin
44/5.6
55,520/5.35
567
19
1.2
0.045
33
gi|7106439
Tubulin beta-5 chain
41/4.9
50,095/4.78
426
12
0.7
0.031
Energy metabolism
27
gi|13879366
NADH dehydrogenase (ubiquinone) Fe-S protein 1
59/5.4
80,724/5.51
264
10
0.9
0.002
28
gi|23272966
Atp5b protein, partial
39/5.0
56,632/5.24
535
22
0.8
0.007
29
gi|23272966
Atp5b protein, partial
39/4.9
56,632/5.24
470
19
0.8
0.008
Glycolysis
26
gi|7305027
Gamma-enolase isoform 1
36/5.0
47,609/4.99
449
14
0.9
0.044
34
gi|7305027
Gamma-enolase isoform 1
36/4.8
47,609/4.99
249
11
0.6
0.004
Antiapoptosis
21
gi|15809030
Beta-synuclein
16/4.2
14,043/4.38
122
29
1.3
0.024
Calcium ion binding
31
gi|34098931
Calretinin
22/4.8
31,467/4.94
199
15
0.8
0.008
Heme metabolic process
30
gi|4886904
Heme-binding protein
20/5.2
21,153/5.18
170
26
0.8
0.004
Protease inhibitor
24
gi|84794552
Phosphatidylethanolamine-binding protein 1
19/5.1
20,988/5.19
203
32
0.9
0.007
Protein degradation
25
gi|6753490
COP9 signalosome complex subunit 4
31/5.8
46,541/5.57
62
4
0.9
0.035
Protein deubiquitination
32
gi|7578956
Ubiquitin C-terminal hydrolase L3
22/4.8
26,333/5.08
113
9
0.8
0.008
Unknown
20
gi|74212109
Unnamed protein product
29/5.5
50,076/4.75
246
10
1.3
0.041
Spot numbers correspond to the 2-DE gel in Figure 1(b). Accession numbers were obtained from the National Center for Biotechnology Information (NCBI) database. Score and sequence coverage (SC) were obtained by Mascot database searching (http://www.matrixscience.com). values were obtained by ANOVA, .
