Metformin Inhibits Mouse Islet Insulin Secretion and Alters Intracellular Calcium in a Concentration-Dependent and Duration-Dependent Manner near the Circulating Range

Figure 1

Metformin exposure reduces insulin accumulation in media in a concentration- and duration-dependent manner. Media were collected from islets exposed to varying concentrations of metformin at 1-day intervals following 1, 2, or 3 days of exposure conducted in three separate trials. Insulin accumulation during these time periods was measured by ELISA. (a) Insulin secretion (Mean +/− SEM) for each concentration of metformin on the x-axis is displayed sequentially over time. (b) 3D plot of the same data to display both the time-dependent (back-to-front) and concentration-dependent (left-to-right) effects of metformin. Sets of 20 size-matched islets were used for each replicate. Numbers above each column indicate the number of replicates. .

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(b)