Novel Mechanisms Modulating Palmitate-Induced Inflammatory Factors in Hypertrophied 3T3-L1 Adipocytes by AMPK

Figure 4

Effects of A769662 on MCP-1, NF-κB, and MAPK signaling and intracellular TG contents in 24 h palmitate-preloaded 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were exposed to 0.3 mmol/L palmitate (black bar) or ethanol vehicle alone (white bar) in the presence or absence of 25 μmol/L A769662 for 24 h. Lysates were immunoblotted to assess the phosphorylation of AMPK on Thr172 (a), NF-κB on Ser536 (e), ERK1/2 on Thr180/Tyr182 (f), ACC on Ser79 (h), and intracellular MCP-1 (c). The release of MCP-1 was also assessed by ELISA (d). The cellular TG (g) contents were measured and then adjusted to intracellular total protein contents. The levels of MCP-1 mRNA (b) were measured by quantitative real-time RT-PCR at 12 h after stimulation and then normalized over the 18S rRNA signal. Data are means ± SEM (). , compared to corresponding control cells.

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