Astragalus membranaceus and Panax notoginseng, the Novel Renoprotective Compound, Synergistically Protect against Podocyte Injury in Streptozotocin-Induced Diabetic Rats

Figure 3

Effects of AG and NG on the expression of WT1, nephrin, and α-dystroglycan detected by immunohistochemical staining. Representative photomicrographs of immunostaining for WT1, nephrin, and α-dystroglycan (a) in the glomeruli of kidney sections. Quantitative analyses of changes in the number of podocytes per glomerular volume (b). The number of podocytes per glomerulus was determined by counting the number of WT1-expressing nuclei per glomerulus. Semiquantitative analyses of immunostaining for nephrin (c) and α-dystroglycan (d) per glomerulus. Control: normal control rats; DN: STZ-induced diabetic rats; losartan: DN rats treated with losartan; AG: DN rats treated with Astragalus membranaceus; NG: DN rats treated with Panax notoginseng; AG+NG: DN rats treated with Astragalus membranaceus plus Panax notoginseng. Results were expressed as the . vs. control group, vs. DN group, ^▲ vs. AG group, ^▼ vs. NG group.

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