Von Hippel-Lindau (VHL) Protein Antagonist VH298 Improves Wound Healing in Streptozotocin-Induced Hyperglycaemic Rats by Activating Hypoxia-Inducible Factor- (HIF-) 1 Signalling

Figure 2

In vitro angiogenesis assay and simulation of wound healing. (a) Angiogenesis measured by the tube formation assay using hUVEC. Tube formation results showed that 30 μM VH298 formed most meshes and master segments and least isolated segments, thereby suggesting that VH298 promotes angiogenesis at low doses but suppresses angiogenesis at high doses, basing on 100 and 200 μM VH298 harming most meshes and master segment formation. Graphs represent (VH298-treated vs. control) (). (b) Scratch test using rFb. 30 μM VH298 significantly accelerated rFb migration after 24 and 48 h observations, and 100 μM VH298 accelerated rFb migration statistically significant only after 24 h observation. Graphs represent (VH298-treated vs. control) (). , , and . n.s.: not significant.

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