High Glucose with Insulin Induces Cell Cycle Progression and Activation of Oncogenic Signaling of Bladder Epithelial Cells Cotreated with Metformin and Pioglitazone

Figure 3

The effect of high glucose with insulin on cell cycle regulator protein p21 and p15/16 in diabetes drug-treated HBlEpC. The cells ( cells/mL) treated with metformin (M) or pioglitazone (P) and cotreated with both drugs were cultured with bladder epithelial cell growth medium containing low glucose or high glucose or high glucose with insulin in six-well plates for 3 days or 7 days. After treatment, the cells were harvested at indicated time and then immunoblotting for p21 and p15/16 were carried out. Results are representative of three independent experiments.

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