High Glucose with Insulin Induces Cell Cycle Progression and Activation of Oncogenic Signaling of Bladder Epithelial Cells Cotreated with Metformin and Pioglitazone

Figure 5

The activity of MAPK in diabetes drug-treated HBlEpC under high glucose with insulin condition. The cells ( cells/mL) cotreated with metformin (M) and pioglitazone (P) were cultured with RPMI-1640 containing low glucose or high glucose or high glucose with insulin in six-well plates for 3 days or 7 days. The cells were harvested, and then western blots for MAPK were performed. Results are representative of three independent experiments.