Effect of Baoshenfang Formula on Podocyte Injury via Inhibiting the NOX-4/ROS/p38 Pathway in Diabetic Nephropathy
Effect of BSF on nephrin expression in podocyte in vivo and in vitro. (a) Representative band of nephrin protein in the cortex of rats. (b) Representative band of nephrin protein in cultured podocyte. (c) Comparison of the grey values of nephrin protein in podocyte of rats. (d) Comparison of mRNA levels of nephrin in podocyte of rats. (e) Comparison of the grey values of nephrin protein in cultured podocyte. (f) Comparison of mRNA levels of nephrin in cultured podocyte. Nephrin mRNA or protein level was significantly decreased in diabetic rats and high glucose (HG) cultured podocytes. BSF treatment significantly increased nephrin expression. , versus normal control (NC), and versus DM or HG.