The Lysine Demethylase KDM5B Regulates Islet Function and Glucose Homeostasis

Figure 1

In vivo administration of GSK-J4. (a-f) Multiple low doses of streptozotocin (STZ) were employed to induce autoimmune diabetes in C57BL/6 mice (vehicle , GSK-J4 ). (a) Body weight (g). (b) Representative images of pancreatic sections stained for insulin, DAPI, and H3K4me3 (). Images are from two different mice. (c) Quantification of the H3K4me3 signal. (d) Quantification of the insulin signal. (e) Glycemic levels from day 0 to day 17. (f) AUC of glycemic levels. (g) Diabetes incidence as determined by hyperglycemia following adoptive transfer of diabetogenic splenocytes from overtly diabetic NOD mice into NODscid mice (vehicle , GSK-J4 ). (h, i) Glycemia and insulin levels measured in C57BL/6 mice 3 days after 1st GSK-J4 () or vehicle () injection. Results are shown as . Statistical significance was determined using two-way ANOVA or unpaired -test. .

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