The Lysine Demethylase KDM5B Regulates Islet Function and Glucose Homeostasis

Figure 3

Phenotypic characterization of young KDM5B-KO mice. (a) Glucose-stimulated insulin secretion was measured ex vivo in isolated islets from WT and KO mice (). (b, c) OGTT. Female mice () aged approximately 9 weeks were fasted overnight (16-18 hours) with access to water ad libitum. Blood samples were taken from a tail puncture at the indicated time points for measurement of glucose. (b) For measurement of insulin, a blood sample was taken from orbital sinus at time points -30 and 15 min for each genotype during OGTT. (c) Glucose excursion curves are shown for each genotype. AUC is shown for comparison of differences in glucose excursions. (d) ITT. Female mice () aged approximately 9 weeks were fasted 2 hours prior to ITT with access to water ad libitum. Insulin was injected i.p. at dosage 0.75 U/kg body weight. (e) Body weight (g) (). Results are shown as . Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparisons test. , .

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