The Lysine Demethylase KDM5B Regulates Islet Function and Glucose Homeostasis

Figure 4

Phenotypic characterization of KDM5B-KO mice aged 30-35 weeks. (a) Body weight (g) (). (b) Body length was measured between nose tip and tail root in anesthetized female mice () of 30-35 weeks of age. (c) Femoral length (cm) () and representative image from a WT and a KO. (d) Plasma GH levels (). (e) Pituitary GH levels (). (f, g) Liver weight () and plasma IGF-1 levels () were measured in female mice of age 37-42 weeks. (h, i) OGTT. Female mice () aged 30-35 weeks were fasted overnight (16-18 hours) with access to water ad libitum. Blood samples were taken from a tail puncture at the indicated time points for measurement of glucose. (h) For measurement of insulin, a blood sample was taken from orbital sinus at time points -30 and 15 min for each genotype during OGTT. (i) Glucose excursion curves are shown for each genotype. AUC is shown for comparison of differences in glucose excursions. (j) ITT. Female mice () aged 30-35 weeks were fasted 2 hours prior to ITT with access to water ad libitum. Insulin was injected i.p. at dosage 0.75 U/kg body weight. (k-n) Protein expression levels of Akt and GSK-3β as determined by quantification of western blots on muscle samples from female mice () of age 37-42 weeks. Results are shown as . Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparison test. Results are shown as . Statistical significance was determined by unpaired -test, one-way or two-way ANOVA. , , and .

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