Advanced Glycation End-Products and Hyperglycemia Increase Angiopoietin-2 Production by Impairing Angiopoietin-1-Tie-2 System

Figure 4

AGEs and HG increased expression of Ang-2. HMEC-1 cells were cultured for 5 days in standard medium (CTR), in the presence of glycated serum (AGEs), 25 mM glucose (HG), and with their combination (AGEs+HG). Western blot analysis of nuclear (a) and cytosolic (b) localization of FoxO1. Histone H3 and GAPDH amounts were analyzed as loading controls. (c) Graph represents relative ratio between nuclear and cytosolic fractions (, and vs. CTR). (d) Western immunoblotting of Ang-2. Representative western blot analysis and quantification of densitometries of western blot band. Data were expressed as of fold induction relative to β-actin. Values shown indicate the mean SEM of at least 3 independent experiments (, , and vs. CTR).

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