FOXO1 Mediates Advanced Glycation End Products Induced Mouse Osteocyte-Like MLO-Y4 Cell Apoptosis and Dysfunctions

Figure 1

AGE treatment induces MLO-Y4 cell apoptosis and dysfunction. (a) MLO-Y4 cells were incubated for BSA or AGE for up to three days and cell viability was detected by MTT assay at 0 h, 24 h, 48 h, and 72 h. (b) Annexin V/PI assays were performed to detect AGE’s role on MLO-Y4 cell apoptosis. Western blot was performed to examine the protein expression of sclerostin and RANKL. (c) Western blot representative images. (d) Quantifications of sclerostin-relative protein expression. (e) Quantifications of RANKL-relative protein expression. qPCR was performed to examine the mRNA expression of sclerostin and RANKL. (f) Quantifications of sclerostin-relative mRNA expression. (g) Quantifications of RANKL-relative mRNA expression. All error bars are standard deviation of the mean. compared to the BSA control group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)