FOXO1 Mediates Advanced Glycation End Products Induced Mouse Osteocyte-Like MLO-Y4 Cell Apoptosis and Dysfunctions

Figure 4

FOXO1 plays as a key regulator of AGE-induced MLO-Y4 cell apoptosis and dysfunction. MLO-Y4 cells were transfected with scramble siRNA or siFOXO1 to detect FOXO1 silencing’s effect on AGE-induced MLO-Y4 cell apoptosis and dysfunction. (a) Annexin V/PI assays were performed to detect apoptosis. qPCR was performed to examine the effect of FOXO1 silencing on expression of caspase-3, sclerostin, and RANKL. (b) Quantifications of caspase-3-relative mRNA expression. (c) Quantifications of sclerostin-relative mRNA expression. (d) Quantifications of RANKL-relative mRNA expression. Western blot was performed to detect cleaved caspase-3, sclerostin, and RANKL protein expressions. (e) Western blot representative images (upper GAPDH is for cleaved caspase-3, bottom GAPDH is for sclerostin and RANKL). (f) Quantifications of cleaved caspase-3-relative expression. (g) Quantifications of sclerostin-relative expression. (h) Quantifications of RANKL-relative expression. All error bars are standard deviation of the mean. compared to relative scramble siRNA control.

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