Melatonin Affects Mitochondrial Fission/Fusion Dynamics in the Diabetic Retina

Figure 1

High glucose induces changes in mitochondrial fission/fusion dynamics in cultured 661W cells. Cultured 661W cells were acutely exposed to high-glucose conditions (HG; 30 mM) for 4, 6, 16, or 24 h (a-d) and collected for Western blotting of DRP1 (b), MFN2 (c), and MCU (d). The control (c) cells were treated with H₂O. (e-g) Cultured 661W cells were treated with 0.01% ethanol (vehicle) as the control (CON), melatonin (MEL, 100 μM; dissolved in 0.01% ethanol), HG (30 mM), or a combination of melatonin and HG (HG+MEL) for 24 h. Cells were then collected and processed for Western blotting analysis of DRP1 (f), MFN2 (g), and MCU (h). The experiments were repeated at least four times (-5). .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)