Islet Transplantation Attenuating Testicular Injury in Type 1 Diabetic Rats Is Associated with Suppression of Oxidative Stress and Inflammation via Nrf-2/HO-1 and NF-κB Pathways
Islet transplantation treatment inhibited NF-κB expression in diabetic rats. (a, b) NF-κB levels measured by immunohistochemical staining (×400) (bar = 25 μm) and quantitative analysis of positive regions showed that the increase in NF-κB was significantly improved in the INS group compared with that in the control group; IT attenuated the expression of the protein ( for each group). (c, d) Western blot images of NF-κB and quantitative analysis of its expression levels in the DM group revealed significant upregulation. By contrast, NF-κB in the IT group was significantly downregulated compared with that in the INS group ( for each group). (e–h) Typical Western blot images of TNF-α, IL-1β, and IL-6 intesticular tissues and quantitative analysis of their expression levels. DM caused a significant increase in the above parameters. The IT treatment group significantly reduced the expression of TNF-α, IL-1β, and IL-6. The expression of the above proteins in the INS group was only slightly altered ( for each group). DM: diabetes mellitus; INS: insulin treatment; IT: islet transplantation. vs. control; & vs. DM; + vs. INS.
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