FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic β-Cells

Figure 3

FXR regulates ADCY8 expression in pancreatic β-cells. mRNA (a) and protein (b) level of ADCY8 in FXR knockdown INS-1 832/13 cells. Data are . ; . mRNA (c) and protein (d) level of ADCY8 in FXR knockout mouse islets. 100-150 islets were used in each experiment. Data represent . ; . mRNA (e) and protein (f) expression of ADCY8 in FXR-overexpressed INS-1 832/13 cells. Data are . ; . mRNA (g) and protein (h) expression of ADCY8 in INS-1 832/13 cells after 24 h treatment with 5 μM GW4064. Data are . ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)