FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic β-Cells

Figure 5

FXR mediates SRC1 binding and histone H3 acetylation at the promoter of Adcy8. ChIP assay was performed to detect the binding of SRC1 (a) and histone H3 acetylation (b) at the Adcy8 promoter in INS-1 832/13 cells treated with 5 μM GW4064 for 24 h. Data represent . ; . FXR knockdown INS-1 832/13 cells were subjected ChIP assay to test SRC1 binding (c) and histone H3 acetylation (d) at the Adcy8 promoter. Data are . ; , . mRNA (e) and protein (f) expression of ADCY8 in scramble or SRC1 knockdown INS-1 832/13 cells treated with 5 μM GW4064 for 24 h. Data are . ; .

(a)

(b)

(c)

(d)

(e)

(f)