FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic β-Cells

Figure 6

Effect of forskolin on insulin secretion and cAMP production in pancreatic β-cells. (a) Insulin secretion of islets isolated from FXR^+/+ and FXR^-/- mice. The islets were stimulated with 2.8 mM glucose or 16.8 mM glucose in the presence or absence of 10 μM forskolin for 30 min. (b) Fold increase of forskolin-potentiated insulin secretion in FXR^+/+ and FXR^-/- mouse islets. Data are . ; , . (c) Insulin secretion of scramble and shFXR INS-1 832/13 cells treated with the condition same with (a). (d) Fold increase of forskolin-potentiated insulin secretion in scramble and shFXR INS-1 832/13 cells. Data represent . ; , . cAMP production in islets from FXR^+/+ and FXR^-/- mice (e) and scramble and shFXR INS-1 832/13 cells (f) incubated with 16.8 mM glucose in the presence or absence of 10 μM forskolin for 30 min. Data are . of each group. , .

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