A Novel Liposome Formulation Carrying Both an Insulin Peptide and a Ligand for Invariant Natural Killer T Cells Induces Accumulation of Regulatory T Cells to Islets in Nonobese Diabetic Mice

Figure 3

Autoantigen-loaded α-GalCer liposome treatment enhances the protective activity of regulatory cells and increases Foxp3⁺ mRNA expression in islets. (a) Diabetogenic CD62L^– T cells were cotransferred into three NOD-SCID recipients, and splenocytes were collected from NOD mice 1 week after the last injection of the following treatments: RGI-3100-iB (□; ), Lipo-GC-TT (△; ), or vehicle (injection○; ). (b) qPCR analyses of Foxp3 transcripts in islets collected from NOD mice subjected to the indicated treatments. Data are represented as mean obtained in three independent experiments, each performed with three pooled mice. Student’s -test, compared with the vehicle treatment.

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