A Liquid-Based Cytology System, without the Use of Cytocentrifugation, for Detection of Podocytes in Urine Samples of Patients with Diabetic Nephropathy

<div>(a) Urinary podocytes were incubated with anti-human podocalyxin monoclonal antibody (22A4), followed by incubation with fluorescein isothiocyanate- (FITC-) labeled rabbit-anti-mouse IgG antibody and observation under a fluorescence microscope. (b) In addition, the same podocytes were subjected to nuclear staining using 4<span class="nowrap"><svg height="11.4859pt" id="M32" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>,</span>6-diamidino-2-phenylindole (DAPI) (original magnification ×800).</div>

Journal of Diabetes Research

fig2

Figure 2

Figure 2: A Liquid-Based Cytology System, without the Use of Cytocentrifugation, for Detection of Podocytes in Urine Samples of Patients with Diabetic Nephropathy 

Figure 2 | A Liquid-Based Cytology System, without the Use of Cytocentrifugation, for Detection of Podocytes in Urine Samples of Patients with Diabetic Nephropathy 