The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice

Table 1

Primers for RT-PCR.

Target genes	Primer sequence (5-3)		Size (bp)
	Forward	Reverse
TSHR	CAGGGTGGAGACGCACACTC	AGAGAGTCCCACAACAGTC	590
TSHR mutant	AGAGAGTCCCACAACAGTC	TCCTTGAAGAAGATGGTGCG	173
Bcl2	GGCATCTTCTCCTTCCAG	ATCCCAGCCTCCGTTAT	441
Bax	GTTACAGGGTTTCATCCAGG	CGTGTCCACGTCAGCAAT	183
Pdx1	TGGGCAGGAGGTGCTTACA	TTCCACTTCATGCGACGGTT	206
Nkx6.1	GACAGCAAATCTTCGCCCTG	ACCAGACCTTGACCTGACTC	117
Insulin	CACCCCACCTGGAGACCTTA	TGAAACAATGACCTGCTTGCTG	153
Glut2	GGGACTTGTGCTGCTGGATA	GAACACGTAAGGCCCAAGGA	244
Ki67	TCACCTGGTCACCATCAAGC	TCAATACTCCTTCCAAACAGGCA	87
GAPDH	CATCAAGAAGGTGGTGAAGC	CATCGAAGGTGGAAGAGTGGG	119