Research Article
Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene
Figure 2
Identification of the m.15897G>A mutation in the tRNA gene. (a) Partial sequence chromatograms of tRNA gene from the proband and a Chinese control. An arrow indicates the location of the base changes at position 15897. (b) The location of the m.15897G>A mutation in the mitochondrial tRNAThr. Arrow indicates the position of the m.15897G>A mutation. (c) Northern blot analysis of tRNAs under native conditions. Two μg of total mitochondrial RNA from various cell lines was electrophoresed through native polyacrylamide gel, electroblotted and hybridized with DIG-labeled oligonucleotide probes specific for the tRNAThr, tRNAIle, and tRNAHis, respectively.
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