Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNA^Thr Gene

Figure 3

Northern blot analysis of mitochondrial tRNA. (a) Equal amounts of total mitochondrial RNA from various cybrid cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with DIG-labeled oligonucleotide probes specific for the tRNA^Ala, tRNA^Ile, tRNA^His, tRNA^Gln, and tRNA^Ala, respectively. (b) Quantification of tRNA levels. Average relative tRNA content per cell was normalized to the average content per cell of 5S rRNA in three mutant cybrid cell lines carrying the m.15897G>A variant and three control cybrid cell lines. The values for the latter are expressed as percentages of the average values for the control cell lines. indicates the significance, according to the -test, of the differences between mutant and control cell lines.

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