Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNA^Thr Gene

Figure 5

Western blot analysis of mitochondrial proteins. (a) 20 μg of total cellular proteins from various cybrid cell lines was electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with respiratory complex subunits in mutant and control cells with VDAC as a loading control. (b) Quantification of 6 respiratory complex subunits. The levels of ND6, ND4, ATP6, CO2, and ND1 in two mutant cell lines and two control cybrid cell lines were determined. The error bars indicate two standard errors of the means. and indicate the significance, according to the -test, of the differences between mutant and control cybrid cell lines.

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