Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNA^Thr Gene

Figure 6

Imbalances in OXPHOS complexes. (a) The steady-state levels of five OXPHOS complexes by Blue Native gel electrophoresis. Fifteen μg of mitochondrial proteins from mutant and control cell lines was electrophoresed through a Blue Native gel, electroblotted and hybridized with antibodies for NDUFA9, SDHA, UQCRC2, COX5A, ATP5C (complexes I, II, III, IV, and V), and VDAC as a loading control. (b) Quantification of levels of complexes I, II, III, IV, and V in two mutant cell lines and two control cybrid cell lines was determined. The error bars indicate two standard errors of the means. and indicate the significance, according to the -test, of the differences between mutant and control cybrid cell lines.

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