High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression

Figure 3

SET domain-containing protein 8 (SETD8) downregulation induced cyclooxygenase 2 (COX2) and inducible nitric oxide synthase (iNOS) expression via upregulation of E26 transformation-specific sequence transcription factor-1 (ESE-1) in human umbilical vein endothelial cells (HUVECs). (a, b) Western blot analysis of SETD8, ESE-1, iNOS, and COX2 expression in HUVECs with SETD8 and ESE-1 knockdown. siESE-1 inhibited shSETD8-induced iNOS and COX2 protein expression in HUVECs. (c) qPCR analysis of SETD8, ESE-1, iNOS, and COX2 expression in HUVECs with SETD8 and ESE-1 knockdown. siESE-1 inhibited shSETD8-induced iNOS and COX2 mRNA expression in HUVECs. (d) Cell apoptosis analysis in HUVECs with SETD8 and ESE-1 knockdown. siESE-1 alleviated shSETD8-mediated cell apoptosis in HUVECs. shSETD8: short hairpin RNA SETD8; siESE-1: small interfering RNA ESE-1. , compared with the control group; ^#, compared with high glucose treatment.

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