High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression

Figure 6

SET domain-containing protein 8 (SETD8) interacts with specificity protein 1 (SP1) to regulate E26 transformation-specific sequence transcription factor-1 (ESE-1) expression in hyperglycaemic HUVECs. (a) H4K20me1 is enriched at the ESE-1 promoter region. (b) SP1 is enriched at the ESE-1 promoter region. (c) Luciferase reporter assays indicated that SETD8 overexpression not only attenuated ESE-1 promoter activity but also augmented the effect of siSP1a on ESE-1 promoter activity. (d) Schematic representation of the working model. SETD8 OE: SETD8 overexpression; siSP1: small interfering RNA SP1. , compared with the control group.

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