Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure

Figure 1

Prolonged treatment with sitagliptin only weakly changed the TEER of unchallenged iBREC. (a) Structures of DPP-4 inhibitors used. (b) Confluent iBREC grown on porous membrane inserts were exposed to 10 or 1000 nM sitagliptin for up to three days. The transendothelial electrical resistance (TEER), determined as a measure of permeability at indicated time points, was only weakly and inconsistently reduced by the inhibitor. TEER values, normalized in relation to those measured immediately before addition of sitagliptin, are shown as means and standard deviations of data from at least four replicates. Statistical analyses were performed as described in Materials and Methods. compared to control.

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