Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure

Figure 2

Treatment with sitagliptin reduced the cell index of unchallenged iBREC. Cells were cultivated on gold electrodes until confluency was reached and exposed to sitagliptin over three days. The cell index (CI) was determined continuously as a measure of barrier function. Sitagliptin (10-1000 nM) resulted in a persistent, concentration-dependent CI decline starting six to forty hours after addition. (a) CI values, normalized in relation to those measured immediately before addition of sitagliptin, are shown as means and standard deviations of data from at least five wells. (b) Statistical analyses of data gained at indicated time points after addition of sitagliptin were performed as described in Materials and Methods. , , , and compared to control.

(a)

(b)