Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure

Figure 3

Sitagliptin did not modulate the barrier dysfunction induced by VEGF-A. Confluent monolayers of iBREC cultivated on gold electrodes were exposed to 10 or 1000 nM sitagliptin in combination with VEGF-A₁₆₅ over two days. The VEGF-A₁₆₅-induced decline of the CI was not affected by the DPP-4 inhibitor. Normalized CI values are presented as means and standard deviations from at least four wells. Normalization and statistical analyses of data recorded at 48 h after addition of sitagliptin and VEGF-A₁₆₅ were performed as described in Materials and Methods. compared to control.