| | Target | Host and type | Source | Working concentrations |
| | Actin | Mouse, monoclonal | Clone AC-40, Abcam (Cambridge, United Kingdom), ab11003 | WB: 500 ng/ml | | Mouse, monoclonal | Clone 5J11, Abcam, ab190301 or Bio-Techne (Wiesbaden, Germany) NBP2-25142 | WB: 700 ng/ml | | Caveolin-1 | Rabbit, polyclonal | Abcam, ab2910 | WB: 20 ng/ml
IF: 2 μg/ml | | CD9 (tetraspanin-29) | Mouse, monoclonal, IgG2 | Clone IVA50, EXBIO (Vestec, Czech Republic, via BIOZOL, Munich, Germany), 11-354C100 | WB: 40 ng/ml
IF: 10 μg/ml
IP: 1 μg/ml | | CD29 (integrin β1) | Mouse, monoclonal, IgG1 | TS2/16, Thermo Fisher Scientific (Langenselbold, Germany), 14-0299-82 | WB: 170 ng/ml
IF: 4 μg/ml
IP: 1 μg/ml | | Claudin-1 | Rabbit, polyclonal | JAY.8, Thermo Fisher Scientific, 51-9000 | WB: 1 μg/ml | | Claudin-1 | Rabbit, polyclonal | Aviva Systems Biology (BIOZOL, Eching, Germany), ARP33623_P50 | IF: 4 μg/ml | | Claudin-5 | Rabbit, polyclonal | Z43.JK, Thermo Fisher Scientific, 34-1600 | WB: 100 ng/ml
IF: 2.5 μg/ml | | VEcadherin | Rabbit, polyclonal | Cell Signaling Technology B.V. (Frankfurt, Germany), 2158S | WB: 1 : 1000
IF: 1 : 100 | | Control IgG UNLB-IgG | Mouse, monoclonal, IgG1 | Clone 15H6, Southern Biotech (Birmingham, United Kingdom) | IP: 1 μg/ml | | Whole IgG, rabbit | Goat, polyclonal, coupled to HRP | Bio-Rad (Munich), 170-5046 | WB: 1 : 30000 | | Whole IgG, mouse | Goat, polyclonal, coupled to HRP | Bio-Rad, 170-5047 | WB: 1 : 30000 |
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