3β-Hydroxysteroid-Δ24 Reductase (DHCR24) Protects Pancreatic β Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Scavenging Excessive Intracellular Reactive Oxygen Species
DHCR24 overexpression protects MIN6 cells from cell death caused by TM exposure. (a) Immunocytochemical (IC) analysis against c-myc (green fluorescent signal) was used to determine the transduction efficiency of Ad-DHCR24-myc. Scale bar, 100 μm. (b) Sterol contents in MIN6 cells were measured 48 h after infection with the indicated adenoviruses (50 PFU/cell). (). (c) Images of MIN6 cells were acquired with a phase-contrast microscope 24 h after TM exposure (0, 0.5, and 1 μg/ml). Scale bar, 100 μm. (d) Numbers of adherent cells are expressed as percentage of cells in the Ad-LacZ group at 0 μg/ml TM exposure. (); vs. Ad-LacZ.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.