3β-Hydroxysteroid-Δ24 Reductase (DHCR24) Protects Pancreatic β Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Scavenging Excessive Intracellular Reactive Oxygen Species
Downregulation of endogenous DHCR24 promotes MIN6 cell apoptosis caused by ER stress. (a) The mRNA levels of DHCR24 were measured by semiquantitative RT-PCR after infection of MIN6 cells with siControl and siDHCR24. (b) The relative mRNA levels of DHCR24 were normalized to GAPDH and calculated as percentage of the levels in the si(-) group. (); vs. si(-) or siControl. (c) Images of MIN6 cells were taken with a phase-contrast microscope after infection with siControl and siDHCR24 followed by treatment with TM for 24 h. Scale bar, 100 μm. (d) The adherent cell number was calculated as percentage of cells in the si(-) group at 0 h after TM treatment. (); vs. si(-) or siControl.
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