Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes

Figure 5

TSF inhibited actin-cytoskeletal disorganization in AGEs-stimulated primary mice podocytes. (a) Morphology of primary mice podocytes in wound healing assay (scale bars, 100 μm). (b) Time-lapse images of primary mice podocytes (scale bars, 50 μm). Lamellipodia are marked with white arrows and filopodia with black arrows. (c) Representative images of different types of phalloidin staining patterns observed in isolated control podocytes (scale bars, 25 μm). (d) Representative immunofluorescence stained with phalloidin and WT1 showed actin-cytoskeletal disorganization in AGEs-stimulated primary mice podocytes which could be relieved by TSF (scale bars, 50 μm). (e) Quantitative analysis of the percentage of four types of phalloidin staining patterns in (d) by a blinded observer (30 cells evaluated per experiment, experiments). The data were expressed as the of three independent experiments performed in triplicate. ^#, ^## vs. BSA group; , vs. AGEs group. Statistically analyzed via a one-way ANOVA with Dunnett’s correction.

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