Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes

Figure 6

TSF improved TRPC6-dependent Ca²⁺ accumulation-mediated reduction of focal adhesions in AGEs-stimulated primary mice podocytes. (a) Effects of TSF or SAR7334 on intracellular Ca²⁺ level of AGEs-induced primary mice podocyte injury ( experiments). (b) Immunoblotting and quantitative analysis of talin1 ( experiments). (c) Immunoblotting and quantitative analysis of TRPC6 and integrin β1 ( experiments). (d) Representative immunofluorescence stained with talin1 (green) and paxillin (red) (scale bars, 50 μm). The data were expressed as the of three independent experiments performed in triplicate. ^#, ^## vs. BSA group; , vs. AGEs group; ^Δ, ^ΔΔ vs. AGEs + TSF group. Statistically analyzed via a one-way ANOVA with Dunnett’s correction.

(a)

(b)

(c)

(d)