Melatonin Alleviates Glucose and Lipid Metabolism Disorders in Guinea Pigs Caused by Different Artificial Light Rhythms

Figure 7

Effects of melatonin on AMPK signaling pathway-related mRNA and protein expression levels in guinea pig hepatic tissue after treatment, at week 10. (a) AMPKα, PPARα, and CPT1A mRNA expression as revealed by quantitative reverse-transcription PCR. (b) Western blotting of p-AMPKα, AMPKα, PPARα, and CPT1A. p-AMPKα (c), AMPKα (d), p-AMPKα/AMPKα (e), PPARα (f), and CPT1A (g) protein expression was quantitated by densitometry analysis of the bands. Values were normalized against that for GAPDH and analyzed with ImageJ software. C: control group; H: HFD group; 12HL: 12 h light group; 24HL: 24 h light group; M: melatonin group; AMPKα: AMP-activated protein kinase α; p-AMPKα: phosphorylated AMPKα; p-AMPK/AMPK: protein ratio between p-AMPK and total-AMPK; PPARα: peroxisome proliferator-activated receptor-α; CPT1A: carnitine palmitoyl-CoA transferase 1 A; GAPDH: glyceraldehyde-3-phosphate dehydrogenase. The values are expressed as the ( per group). ^# and ^###, HFD vs. control; ^{^^}, 12HL vs. HFD group; ^&, ^&&, and ^&&&, 24HL vs. 12HL group; , M vs. 24HL group.

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